Brain on the stage - spotlight on nervous system development in zebrafish: EMBO practical course, KIT, Sept. 2013

Abstract

During the EMBO course 'Imaging of Neural Development in Zebrafish', held on September 9-15th 2013, researchers from different backgrounds shared their latest results, ideas and practical expertise on zebrafish as a model to address open questions regarding nervous system development.

Figure 1

Mosaic labelling techniques in the zebrafish embryo. (A, C) Visualisation of individual transplanted retinal cells from donor embryos labelled with either H2B-RFP and membrane tagged-GFP (A) or the Atoh7-gap43RFP transgene (C) at 28 hpf. In (C) frames from a time-lapse series showing apical process retraction from the apical surface (up, see vertical dotted line) and axon extension at the basal surface (white arrow) of the s2 sibling that differentiates into a retinal ganglion cell after asymmetric division. R, Retina; L, lens. Scale bar is 10 μm. (B) Few small cell clones labelled by electroporation of CMV:mCitrine. Acquired images were pseudo-coloured afterwards according to z-level to visualize individual cell clones in the brain at 32 hpf. In (A, C), the retina is oriented dorsal up. (B) Dorsal view of the midbrain. The data presented in this figure were acquired by the course participants with the help of Wiebke Sassen (Braunschweig), Shahad Albadri and Anne-Laure Duchemin (Heidelberg).

Figure 2

Imaging of zebrafish gastrulation by digital light sheet microscopy. Maximum-intensity projections of H2B-GFP labelled wild type zebrafish embryo at the indicated times and subsequent developmental stages from A to F. The data presented in this figure were acquired by the course participants with the help of Andrey Kobitski, KIT.