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. 2013 Dec 17;10(12):7310-26.
doi: 10.3390/ijerph10127310.

The association between splenocyte apoptosis and alterations of Bax, Bcl-2 and caspase-3 mRNA expression, and oxidative stress induced by dietary nickel chloride in broilers

Jianying Huang, Hengmin Cui  1 , Xi Peng, Jing Fang, Zhicai Zuo, Junliang Deng, Bangyuan Wu
Affiliations

The association between splenocyte apoptosis and alterations of Bax, Bcl-2 and caspase-3 mRNA expression, and oxidative stress induced by dietary nickel chloride in broilers

Jianying Huang et al. Int J Environ Res Public Health. .

Abstract

Two hundred and forty avian broilers were equally divided into four groups, and raised with a corn-soybean basal diet or the same diet supplemented with 300, 600, 900 mg/kg NiCl2 for 42 days. Numbers or percentages of apoptotic splenocytes by flow cytometry (FCM) and TUNEL were higher (p < 0.05 or p < 0.01) in the 300, 600 and 900 mg/kg groups than those in the control group. Results measured by qRT-PCR and ELISA showed that mRNA expression and contents were significantly higher (p < 0.05 or p < 0.01) in Bax and Caspase-3, and were significantly lower (p < 0.05 or p < 0.01) in Bcl-2 of the 300, 600 and 900 mg/kg groups. Also, the SOD, CAT and GSH-Px activities, and the ability to inhibit hydroxyl radical, and GSH contents were significantly decreased (p < 0.05 or p < 0.01), and MDA contents were increased (p < 0.05 or p < 0.01) in all groups. In conclusion, dietary NiCl2 in excess of 300 mg/kg caused apoptosis, altered Bax, Bcl-2 and Caspase-3 mRNA expression levels and contents, and induced oxidative stress in the spleen. Also, splenocyte apoptosis was closely related to the alternations of Bax, Bcl-2 and Caspase-3 mRNA expression, and oxidative damage. The splenic immunity and blood filtration functions were impaired in broilers.

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Figures

Figure 1
Figure 1
Positive cells stained by TUNEL in the spleen at 14 days of age.
Figure 2
Figure 2
Positive cells stained by TUNEL in the spleen at 28 days of age.
Figure 3
Figure 3
Positive cells stained by TUNEL in the spleen at 42 days of age.
Figure 4
Figure 4
Numbers of positive cells by TUNEL.
Figure 5
Figure 5
Percentage (%) of apoptotic splenocytes by FCM.
Figure 6
Figure 6
mRNA expression levels of Bax, Bcl-2 and Caspase-3 in the spleen.
Figure 7
Figure 7
Changes of Bax, Bcl-2 and Caspase-3 contents in the spleen.
Figure 8
Figure 8
Changes of the oxidative stress parameters in the spleen.
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