Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Dec 17;10(12):7310-26.
doi: 10.3390/ijerph10127310.

The association between splenocyte apoptosis and alterations of Bax, Bcl-2 and caspase-3 mRNA expression, and oxidative stress induced by dietary nickel chloride in broilers

Jianying Huang, Hengmin Cui  1 , Xi Peng, Jing Fang, Zhicai Zuo, Junliang Deng, Bangyuan Wu
Affiliations

The association between splenocyte apoptosis and alterations of Bax, Bcl-2 and caspase-3 mRNA expression, and oxidative stress induced by dietary nickel chloride in broilers

Jianying Huang et al. Int J Environ Res Public Health. .

Abstract

Two hundred and forty avian broilers were equally divided into four groups, and raised with a corn-soybean basal diet or the same diet supplemented with 300, 600, 900 mg/kg NiCl2 for 42 days. Numbers or percentages of apoptotic splenocytes by flow cytometry (FCM) and TUNEL were higher (p < 0.05 or p < 0.01) in the 300, 600 and 900 mg/kg groups than those in the control group. Results measured by qRT-PCR and ELISA showed that mRNA expression and contents were significantly higher (p < 0.05 or p < 0.01) in Bax and Caspase-3, and were significantly lower (p < 0.05 or p < 0.01) in Bcl-2 of the 300, 600 and 900 mg/kg groups. Also, the SOD, CAT and GSH-Px activities, and the ability to inhibit hydroxyl radical, and GSH contents were significantly decreased (p < 0.05 or p < 0.01), and MDA contents were increased (p < 0.05 or p < 0.01) in all groups. In conclusion, dietary NiCl2 in excess of 300 mg/kg caused apoptosis, altered Bax, Bcl-2 and Caspase-3 mRNA expression levels and contents, and induced oxidative stress in the spleen. Also, splenocyte apoptosis was closely related to the alternations of Bax, Bcl-2 and Caspase-3 mRNA expression, and oxidative damage. The splenic immunity and blood filtration functions were impaired in broilers.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Positive cells stained by TUNEL in the spleen at 14 days of age.
Figure 2
Figure 2
Positive cells stained by TUNEL in the spleen at 28 days of age.
Figure 3
Figure 3
Positive cells stained by TUNEL in the spleen at 42 days of age.
Figure 4
Figure 4
Numbers of positive cells by TUNEL.
Figure 5
Figure 5
Percentage (%) of apoptotic splenocytes by FCM.
Figure 6
Figure 6
mRNA expression levels of Bax, Bcl-2 and Caspase-3 in the spleen.
Figure 7
Figure 7
Changes of Bax, Bcl-2 and Caspase-3 contents in the spleen.
Figure 8
Figure 8
Changes of the oxidative stress parameters in the spleen.
See this image and copyright information in PMC

References

    1. Scott-Fordsmand J.J. Toxicity of nickel to soil organisms in Denmark. Rev. Environ. Contam. Toxicol. 1997;148:1–34.
    1. Haber L., Erdreicht L., Diamond G., Maier A., Ratney R., Zhao Q., Dourson M. Hazard identification and dose response of inhaled nickel-soluble salts. Regul. Toxicol. Pharmacol. 2000;31:210–230. doi: 10.1006/rtph.2000.1377. - DOI - PubMed
    1. Diagomanolin V., Farhang M., Ghazi-Khansari M., Jafarzadeh N. Heavy metals (Ni, Cr, Cu) in the Karoon waterway river, Iran. Toxicol. Lett. 2004;151:63–67. doi: 10.1016/j.toxlet.2004.02.018. - DOI - PubMed
    1. Coogan T.P., Latta D.M., Snow E.T., Costa M., Lawrence A. Toxicity and carcinogenicity of nickel compounds. CRC Crit. Rev. Toxicol. 1989;19:341–384. doi: 10.3109/10408448909029327. - DOI - PubMed
    1. Grandjean P. Human exposure to nickel. IARC Sci. Publ. 1984;53:469–485. - PubMed

Publication types

MeSH terms

LinkOut - more resources