Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Dec 20;5(13):3517-23.
doi: 10.1002/j.1460-2075.1986.tb04677.x.

Differential nuclease sensitivity identifies tight contacts between yeast pre-mRNA and spliceosomes

B C Rymond, M Rosbash

Differential nuclease sensitivity identifies tight contacts between yeast pre-mRNA and spliceosomes

B C Rymond et al. EMBO J. .

Abstract

The oligonucleotide-directed RNase H sensitivity of a yeast (Saccharomyces cerevisiae) pre-mRNA was determined in an in vitro splicing reaction. While most of the pre-mRNA was sensitive to cleavage, the regions of the 5' splice site and TACTAAC box were found to be highly resistant. The biochemical requirements for protection against nuclease attack parallel those of both spliceosome formation and splicing. Most of the uncleaved pre-mRNA remaining after RNase H challenge was found associated with two forms of the yeast spliceosome. Differences in the RNase H sensitivity of pre-mRNA found in the two spliceosome forms indicate an increased association of splicing factors with the 5' splice site during spliceosome assembly.

PubMed Disclaimer

References

    1. Cell. 1985 Aug;42(1):335-44 - PubMed
    1. Science. 1985 May 24;228(4702):963-7 - PubMed
    1. Cell. 1985 Oct;42(3):737-50 - PubMed
    1. J Biol Chem. 1985 Nov 25;260(27):14780-92 - PubMed
    1. Nature. 1985 Oct 24-30;317(6039):732-4 - PubMed

Publication types