doi: 10.1128/mcb.7.1.549-551.1987.
A mechanism by which adenovirus virus-associated RNAI controls translation in a transient expression assay
- PMID: 2436038
- PMCID: PMC365102
- DOI: 10.1128/mcb.7.1.549-551.1987
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A mechanism by which adenovirus virus-associated RNAI controls translation in a transient expression assay
Mol Cell Biol.
1987 Jan.
Abstract
The mechanism by which adenovirus virus-associated RNAI stimulates translational efficiency in a transient-expression assay in 293 cells was investigated. We showed that DNA transfection leads to activation of a protein kinase that phosphorylates the alpha subunit of eucaryotic initiation factor 2 and, as a consequence, inhibition of polypeptide chain initiation. Cotransfection of a plasmid encoding adenovirus type 2 virus-associated RNAI recovered the translational capacity by preventing activation of the kinase.
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