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. 1987 Jan;7(1):549-51.
doi: 10.1128/mcb.7.1.549-551.1987.

A mechanism by which adenovirus virus-associated RNAI controls translation in a transient expression assay

G Akusjärvi, C Svensson, O Nygård

A mechanism by which adenovirus virus-associated RNAI controls translation in a transient expression assay

G Akusjärvi et al. Mol Cell Biol. 1987 Jan.

Abstract

The mechanism by which adenovirus virus-associated RNAI stimulates translational efficiency in a transient-expression assay in 293 cells was investigated. We showed that DNA transfection leads to activation of a protein kinase that phosphorylates the alpha subunit of eucaryotic initiation factor 2 and, as a consequence, inhibition of polypeptide chain initiation. Cotransfection of a plasmid encoding adenovirus type 2 virus-associated RNAI recovered the translational capacity by preventing activation of the kinase.

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