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. 2010 Winter;9(1):75-82.

Antioxidant Potential of Crude Extract and Different Fractions of Enhydra fluctuans Lour

Santanu Sannigrahi  1 , Upal Kanti Mazuder  2 , Dilip Kumar Pal  3 , Sambit Parida  1 , Sourabh Jain  1
Affiliations

Antioxidant Potential of Crude Extract and Different Fractions of Enhydra fluctuans Lour

Santanu Sannigrahi et al. Iran J Pharm Res. 2010 Winter.

Abstract

The antioxidant potential of crude methanol extract (CE) as well as chloroform (CF), ethyl acetate (EF) and n-butanol (NF) soluble fractions of Enhydra fluctuans Lour, which is widely used in indigenous system of medicine for different purposes, were studied. The antioxidant potential of extract/different fractions were evaluated using different in vitro antioxidant models. In addition, total amount of polyphenolics compounds, DPPH (1,1-diphenyl-2-picryl hydrazyl) radical, nitric oxide, superoxide anion, hydroxyl radical scavenging activities and reductive power of crude extracted its different fractions were determined. It was found that ethyl acetate fraction have maximum amount of polyphenolics compounds (179.7 ± 18.23 μg / mg pyrocatechol equivalent). This fraction was found more effective than crude extract and other fractions in all the above mentioned assays.

Keywords: Antioxidant activity; Different fractions; Enhydra fluctuans; Flavonoids; Free radical scavenging activity.

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Figures

Scheme 1
Scheme 1
Scheme of extraction of aerial partsof Enhydra fluctuans Lour
Figure 1
Figure 1
Radical scavenging potential of extract/fractions by DPPH method at different concentrations (μg/mL).
Figure 2
Figure 2
Nitric oxide scavenging potential of extract/fractions at different concentrations (μg/mL).
Figure 3
Figure 3
Scavenging potential of extract/fractions at different concentrations (μg/mL) on superoxide radicals generated by the PMS/NADH system
Figure 4
Figure 4
Hydroxy radical scavenging potential of extract/fractions at different concentrations (μg/mL) on deoxyribose degradation method
Figure 5
Figure 5
Reducing power of the extract/fractions by Fe3+ - Fe2+ transformation
See this image and copyright information in PMC

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