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. 2013:2013:810617.
doi: 10.1155/2013/810617. Epub 2013 Nov 28.

Circulation of HIV-1 CRF02_AG among MSM population in central Italy: a molecular epidemiology-based study

Massimo Giuliani  1 , Maria M Santoro  2 , Alessandra Lo Presti  3 , Eleonora Cella  3 , Paola Scognamiglio  4 , Alessia Lai  5 , Alessandra Latini  6 , Lavinia Fabeni  4 , Caterina Gori  4 , Carmela Pinnetti  4 , Enrico Girardi  4 , Carlo F Perno  7 , Gianguglielmo Zehender  5 , Massimo Ciccozzi  3
Affiliations

Circulation of HIV-1 CRF02_AG among MSM population in central Italy: a molecular epidemiology-based study

Massimo Giuliani et al. Biomed Res Int. 2013.

Abstract

Introduction: The evolutionary and demographic history of the circular recombinant form CRF02_AG in a selected retrospective group of HIV-1 infected men who have sex with men (MSM) resident in Central Italy was investigated.

Methods: A total of 55 HIV-1 subtype CRF02_AG pol sequences were analyzed using Bayesian methods and a relaxed molecular clock to reconstruct their dated phylogeny and estimate population dynamics.

Results: Dated phylogeny indicated that the HIV-1 CRF02_AG strains currently circulating in Central Italy originated in the early 90's. Bayesian phylogenetic analysis revealed the existence of a main HIV-1 CRF02_AG clade, introduced in the area of Rome before 2000 and subsequently differentiated in two different subclades with a different date of introduction (2000 versus 2005). All the sequences within clusters were interspersed, indicating that the MSM analyzed form a close and restricted network where the individuals, also moving within different clinical centers, attend the same places to meet and exchange sex.

Conclusions: It was suggested that the HIV-1 CRF02_AG epidemic entered central Italy in the early 1990s, with a similar trend observed in western Europe.

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Figures

Figure 1
Figure 1
Bayesian time-scaled tree of the 55 HIV-1 CRF 02_AG pol sequences from MSM. The numbers at the root and at the internal nodes represent the estimated date of the origin and the uncertainty indicated by 95% highest posterior density (95% HPD) intervals. The asterisks (∗) along a branch represent significant statistical support for the clade subtending that branch (posterior probability >98%). Non-Italian sequences are in bold. The line at the bottom represents time (in years).
Figure 2
Figure 2
Bayesian skyline plot (BSP) of the 55 HIV-1 CRF 02_AG pol sequences from MSM. The effective number of infections is reported on the Y-axis. Time is reported in the X-axis. The colored area corresponds to the credibility interval based on 95% highest posterior density interval (HPD).
See this image and copyright information in PMC

References

    1. Barin F, Courouce A-M, Pillonel J, Buzelay L. Increasing diversity of HIV-1(M) serotypes in French blood donors over a 10-year period (1985–1995) AIDS. 1997;11(12):1503–1508. - PubMed
    1. Weidle PJ, Ganea CE, Irwin KL, et al. Presence of human immunodeficiency virus (HIV) type 1, group M, non-B subtypes, Bronx, New York: a sentinel site for monitoring HIV genetic diversity in the United States. Journal of Infectious Diseases. 2000;181(2):470–475. - PubMed
    1. Yerly S, Vora S, Rizzardi P, et al. Acute HIV infection: impact on the spread of HIV and transmission of drug resistance. AIDS. 2001;15(17):2287–2292. - PubMed
    1. Frange P, Galimand J, Vidal N, et al. New and old complex recombinant HIV-1 strains among patients with primary infection in 1996–2006 in France: the French ANRS CO06 Primo Cohort Study. Retrovirology. 2008;5, article 69 - PMC - PubMed
    1. Holguín A, de Mulder M, Yebra G, López M, Soriano V. Increase of non-B subtypes and recombinants among newly diagnosed HIV-1 native spaniards and immigrants in Spain. Current HIV Research. 2008;6(4):327–334. - PubMed

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