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. 1987 May;76(1):37-9.
doi: 10.1007/BF00283047.

Sequential G- to R-banding for high resolution chromosome analysis

B Hirsch, R Mack, D Arthur

Sequential G- to R-banding for high resolution chromosome analysis

B Hirsch et al. Hum Genet. 1987 May.

Abstract

A sequential staining protocol for revealing first G- and then R-bands on early metaphase chromosomes is presented. Lymphocyte cultures are synchronized with methotrexate, and released from the S-phase block with bromodeoxyuridine. The resulting early metaphase cells are initially G-banded with Wright's stain and then R-banded by the fluorescence plus Giemsa (FPG) technique.

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