Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Sep 1;1(1):53-9.
doi: 10.1016/j.rinim.2011.08.004. eCollection 2011.

Peritoneal macrophages from patients with cirrhotic ascites show impaired phagocytosis and vigorous respiratory burst

Abdel Motaal M Ahmed  1 , Adrian Bomford  1 , Kayhan T Nouri-Aria  1 , Ted Davies  1 , Roger Smith  1 , Roger Williams  2
Affiliations

Peritoneal macrophages from patients with cirrhotic ascites show impaired phagocytosis and vigorous respiratory burst

Abdel Motaal M Ahmed et al. Results Immunol. .

Abstract

Cirrhotic patients (CPs) are susceptible to spontaneous bacterial peritonitis (SBP). Aim of this study was to examine if this susceptibility was related to peritoneal macrophages' (PMs) altered host defence. Absorbance of phagocytosed particles by PMs from CPs was lower than that of control (31.88% vs. 77.2%). Particle opsonisation increased the absorbance to 41% in CPs' PMs, and this value remains lower than the control; 77.2%. Respiratory burst (RB) was expressed as fluorescence index values, and these were higher in PMs from CPs than in controls (82 vs. 41, 73 vs. 26 and 71 vs. 26). IFN-γ made no further increase of RB values in PMs from CPs. CD14 expression was also higher in CPs' PMs. IFN-γ significantly downregulated CD14 expression in both CPs' PMs and control. Reduced phagocytosis by predominantly CD14-positive PMs from CPs could be related to intense RB. Findings suggest altered host defence that could contribute to susceptibility to SBP.

Keywords: CD14 expression; Peritoneal macrophages; Respiratory burst; Spontaneous bacterial peritonitis; Yeast phagocytosis.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Phagocytosis measurement in patient and control PMs.
Fig. 2
Fig. 2
CD14 phenotype expression in patient's PM (dark bars) and controls (light bars) before and after INF-γ treatment.
Fig. 3
Fig. 3
Measurement of Respiratory Burst (RB) in patients' PM (dark bars) and controls (light bars) PM.
Fig. 4
Fig. 4
Effect of Interferon-γ (IFN-γ) on Respiratory Burst (RB) in patient and control PMs.
See this image and copyright information in PMC

References

    1. Garcia-Tsao G., Albillos A., Barden G.E., West A.B. Bacterial translocation in acute and chronic portal hypertension. Hepatology. 1993;17(6):1081–1085. - PubMed
    1. Rajkovic I.A., Williams R. Abnormalities of neutrophil phagocytosis, intracellular killing and metabolic activity of alcoholic cirrhosis and hepatitis. Hepatology. 1986;6:252–262. - PubMed
    1. Hassner A., Kletter Y., Schlag D., Yedvab M., Aronson M., Shibolet S. Impaired monocyte function in liver cirrhosis. British Medical Journal. 1981;282:1262–1263. - PMC - PubMed
    1. Wyke R.J., Rajkovic I.A., Williams R. Impaired opsonization by serum from patients with chronic liver disease. Clinical and Experimental Immunology. 1983;51:91–98. - PMC - PubMed
    1. Simberkoff M.S., Moldover N.H., Weiss G. Bactericidal and opsonic activity of cirrhotic ascites and non-ascitic fluid. Journal of Laboratory and Clinical Medicine. 1978;91:831–839. - PubMed