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. 2014 May;36(5):1037-41.
doi: 10.1007/s10529-013-1431-z. Epub 2013 Dec 29.

Mature embryo-derived wheat transformation for tolerance to moisture stress

Lakshmi Kasirajan  1 , Boomiraj Kovilpillai, Kailash Chander Bansal
Affiliations

Mature embryo-derived wheat transformation for tolerance to moisture stress

Lakshmi Kasirajan et al. Biotechnol Lett. 2014 May.

Abstract

We have developed marker-free transgenic wheat using a transcription factor, AtDREB1A cloned from Arabidopsis. Southern hybridization confirmed a transgenic event with a single copy insertion. PCR analysis of the T1 plants showed four were positive only for AtDREB1A. A T1 plant (HRCB3#17-37) was marker-free and had good expression of drought tolerance in comparison with untransformed plants. The leaf relative water content of this T1 transgenic plant was 12-15% higher than that of the wild type during stress with an 8% higher yield under water deficit conditions compared with wild type plants.

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