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. 2013 Dec 20;8(12):e82864.
doi: 10.1371/journal.pone.0082864. eCollection 2013.

First evidence of genetic intraspecific variability and occurrence of Entamoeba gingivalis in HIV(+)/AIDS

Sibeli B S Cembranelli  1 , Fernanda O Souto  1 , Kennio Ferreira-Paim  1 , Túlio T Richinho  1 , Poliana L Nunes  1 , Gabriel A N Nascentes  1 , Thatiana B Ferreira  1 , Dalmo Correia  1 , Eliane Lages-Silva  1
Affiliations

First evidence of genetic intraspecific variability and occurrence of Entamoeba gingivalis in HIV(+)/AIDS

Sibeli B S Cembranelli et al. PLoS One. .

Abstract

Entamoeba gingivalis is considered an oral commensal but demonstrates a pathogenic potential associated with periodontal disease in immunocompromised individuals. Therefore, this study evaluated the occurrence, opportunistic conditions, and intraspecific genetic variability of E. gingivalis in HIV(+)/AIDS patients. Entamoeba gingivalis was studied using fresh examination (FE), culture, and PCR from bacterial plaque samples collected from 82 HIV(+)/AIDS patients. Genetic characterization of the lower ribosomal subunit of region 18S (18S-SSU rRNA) was conducted in 9 positive samples using low-stringency single specific primer PCR (LSSP-PCR) and sequencing analysis. Entamoeba gingivalis was detected in 63.4% (52/82) of the samples. No association was detected between the presence of E. gingivalis and the CD4(+) lymphocyte count (≤200 cells/mm(3) (p = 0.912) or viral load (p = 0.429). The LSSP-PCR results helped group E. gingivalis populations into 2 polymorphic groups (68.3% similarity): group I, associated with 63.6% (7/11) of the samples, and group II, associated with 36.4% (4/11) of the samples, which shared 74% and 83.7% similarity and association with C and E isolates from HIV(-) individuals, respectively. Sequencing of 4 samples demonstrated 99% identity with the reference strain ATCC 30927 and also showed 2 divergent clusters, similar to those detected by LSSP-PCR. Opportunistic behavior of E. gingivalis was not detected, which may be related to the use of highly active antiretroviral therapy by all HIV(+)/AIDS patients. The high occurrence of E. gingivalis in these patients can be influenced by multifactorial components not directly related to the CD4(+) lymphocyte counts, such as cholesterol and the oral microbiota host, which could mask the potential opportunistic ability of E. gingivalis. The identification of the 18S SSU-rRNA polymorphism by LSSP-PCR and sequencing analysis provides the first evidence of genetic variability in E. gingivalis isolated from HIV patients.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. LSSP-PCR. Polyacrylamide gel (7.5%) stained with silver nitrate.
Primers: A) EGO-1 and B) EGO-2. Lane 1: molecular marker 1 Kb (Invitrogen); lanes 2–25: HIV(+)/AIDS samples; lane 26: negative control.
Figure 2
Figure 2. Analysis of the genetic variability of the small ribosomal subunit in the 18S rRNA of E. gingivalis in clinical samples from HIV(+)/AIDS patients, detected by LSSP-PCR.
M = male; F = female; CD4 =  CD4+ cell count; HIV− = HIV negative; UR = unrealized.
Figure 3
Figure 3. Phylogenetic relationship of Entamoeba spp. from HIV infected patients.
Neighbor-joining method was performed in 24 taxa at the SSU rRNA locus. The optimal tree with the sum of branch length = 1.32 is shown and the bootstrap values were added to phylogenetic branches.
Figure 4
Figure 4. Single nucleotide polymorphisms in E. gingivalis isolates.
See this image and copyright information in PMC

References

    1. WHO (1993) Classification and diagnostic criteria for oral lesions in HIV infection. EC-Clearinghouse on Oral Problems Related to HIV Infection and WHO Collaborating Centre on Oral Manifestations of the Immunodeficiency Virus. J Oral Pathol Med 22: 289–291. - PubMed
    1. Gros G (1849) Fragments d’helminthologie et de physiologie microscopique. Bull Soc Imp Nat Moscou 22: 549–573.
    1. Cuevas RF, Ramos MAS, Contreras CC, Hernández-Sierra F (2008) Prevalencia y asociación epidemiológica de los protozoarios orales Entamoeba gingivalis y Trichomonas tenax en niños mexicanos. Rev ADM 65: 259–262.
    1. Ghabanchi J, Zibaei M, Afkar MD, Sarbazie AH (2010) Prevalence of oral Entamoeba gingivalis and Trichomonas tenax in patients with periodontal disease and healthy population in Shiraz, southern Iran. Indian J Dent Res 21: 89–91. - PubMed
    1. Lucht E, Evengard B, Skott J, Pehrson P, Nord CE (1998) Entamoeba gingivalis in human immunodeficiency virus type 1-infected patients with periodontal disease. Clin Infect Dis 27: 471–473. - PubMed

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