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. 2013 Dec 20;8(12):e85307.
doi: 10.1371/journal.pone.0085307. eCollection 2013.

Identification of novel QTLs for isolate-specific partial resistance to Plasmodiophora brassicae in Brassica rapa

Jingjing Chen  1 , Jing Jing  1 , Zhongxiang Zhan  2 , Teng Zhang  1 , Chunyu Zhang  2 , Zhongyun Piao  1
Affiliations

Identification of novel QTLs for isolate-specific partial resistance to Plasmodiophora brassicae in Brassica rapa

Jingjing Chen et al. PLoS One. .

Abstract

Plasmodiophora brassicae, the causal agent of clubroot disease of the Brassica crops, is widespread in the world. Quantitative trait loci (QTLs) for partial resistance to 4 different isolates of P. brassicae (Pb2, Pb4, Pb7, and Pb10) were investigated using a BC1F1 population from a cross between two subspecies of Brassica rapa, i.e. Chinese cabbage inbred line C59-1 as a susceptible recurrent parent and turnip inbred line ECD04 as a resistant donor parent. The BC1F2 families were assessed for resistance under controlled conditions. A linkage map constructed with simple sequence repeats (SSR), unigene-derived microsatellite (UGMS) markers, and specific markers linked to published clubroot resistance (CR) genes of B. rapa was used to perform QTL mapping. A total of 6 QTLs residing in 5 CR QTL regions of the B. rapa chromosomes A01, A03, and A08 were identified to account for 12.2 to 35.2% of the phenotypic variance. Two QTL regions were found to be novel except for 3 QTLs in the respective regions of previously identified Crr1, Crr2, and Crr3. QTL mapping results indicated that 1 QTL region was common for partial resistance to the 2 isolates of Pb2 and Pb7, whereas the others were specific for each isolate. Additionally, synteny analysis between B. rapa and Arabidopsis thaliana revealed that all CR QTL regions were aligned to a single conserved crucifer blocks (U, F, and R) on 3 Arabidopsis chromosomes where 2 CR QTLs were detected in A. thaliana. These results suggest that some common ancestral genomic regions were involved in the evolution of CR genes in B. rapa.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Genetic linkage map of Brassica rapa.
Linkage groups were numbered A1 to A10 according to the anchor markers. The distances in centiMorgan were given on the left and the marker numbers are indicated on the right. The newly mapped markers were marked with boldface and asterisks. The markers linked to published clubroot resistance genes were underlined.
Figure 2
Figure 2. Frequency distributions of the disease index for clubroot resistance to the 4 isolates of Plasmordium brassicae in BC1F2 families.
Figure 3
Figure 3. Linkage maps of four Brassica rapa chromosomes with detected QTL for clubroot resistance.
The distances in centiMorgan are given on the left and the marker numbers are indicated on the right. The markers on the peak of each QTL are underlined and marked with boldface. The boxes indicate confidence intervals of QTL detected with the 4 isolates.
Figure 4
Figure 4. Microsynteny of QTL regions for clubroot resistance between Brassica rapa and Arabidopsis thaliana.
The number on the left of vertical bars indicates the physical position in megabase (Mb) of chromosomes either from B. rapa or Arabidopsis. The clubroot resistance (CR) QTL regions are indicated by vertical lines with 2 arrows. The markers linked to CR QTLs in each linkage map are indicated in boldface. The markers linked to each published CR locus are underlined. The Arabidopsis genes or genomic regions (bacterial artificial chromosome clones) corresponding to the markers on the linkage groups of B. rapa are connected by lines with 2 arrows.
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