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. 2013;14(11):6305-9.
doi: 10.7314/apjcp.2013.14.11.6305.

TIAM2 enhances non-small cell lung cancer cell invasion and motility

Zheng-Yuan Zhao  1 , Chen-Guang Han, Jun-Tao Liu, Chang-Lei Wang, Yi Wang, Li-Ya Cheng
Affiliations
Free article

TIAM2 enhances non-small cell lung cancer cell invasion and motility

Zheng-Yuan Zhao et al. Asian Pac J Cancer Prev. 2013.
Free article

Abstract

Background: TIAM2, a Rac guanine nucleotide exchange factor, is closely associated with cell adherence and migration. Here, we aimed to investigate the role of TIAM2 in non-small cell lung cancer (NSCLC) cells.

Materials and methods: A small interference RNA (siRNA) was introduced to silence the expression of TIAM2. Invasion and motility assays were then performed to assess the invasion and motility potential of NSCLC cells. GST-pull down assays were used to detect activation of Rac1.

Results: TIAM2 was highly expressed in NSCLC cells. Knockdown of TIAM2 inhibited the invasion and motility, and suppressed activation of Rac1. Further experiments demonstrated that knockdown of TIAM2 could up-regulate the expression of E-cadherin, and down- regulate the expression of MMP-3, Twist and Snail.

Conclusions: Our data suggest that TIAM2 can promote invasion and motility of NSCLC cells. Activation of Rac1 and regulation of some EMT/invasion-related genes may be involved in the underlying processes.

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