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. 2014 Jan;143(1):29-38.
doi: 10.1085/jgp.201311083.

Cyclic AMP compartments and signaling specificity: role of cyclic nucleotide phosphodiesterases

Marco Conti  1 , Delphine Mika, Wito Richter
Affiliations

Cyclic AMP compartments and signaling specificity: role of cyclic nucleotide phosphodiesterases

Marco Conti et al. J Gen Physiol. 2014 Jan.
No abstract available

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Figures

Figure 1.
Figure 1.
Scheme illustrating the different hypothetical roles of PDEs in the generation of cyclic nucleotide compartments. Three models are presented. (A) A PDE functions as a barrier to diffusion. (B) A PDE functions as a sink generating a domain of low cAMP. (C) Different, slowly equilibrating compartments are present in a cell with PDE concentration varying among compartments. In this latter model, a PDE contributes to generation of compartments by regulating cAMP concentration and cAMP fluxes between compartments.
Figure 2.
Figure 2.
Rate of cAMP decay at the membrane in wild-type and PDE4BKO neonatal cardiac myocytes. Myocytes expressing EPAC2-PM were stimulated with 10 nM isoproterenol, followed by treatment with 1 µM propranolol 80 s later. R/R0 recordings for each cell were transformed into concentrations of cAMP using the equation of Börner et al. (2011). Data were averaged and fitted with an exponential decay equation, and the initial rate of cAMP hydrolysis was calculated from the K constant and initial [cAMP]. Data represent the mean ± SEM of n ≥ 25 cells analyzed.
See this image and copyright information in PMC

References

    1. Anant J.S., Ong O.C., Xie H.Y., Clarke S., O’Brien P.J., Fung B.K. 1992. In vivo differential prenylation of retinal cyclic GMP phosphodiesterase catalytic subunits. J. Biol. Chem. 267:687–690 - PubMed
    1. Bacskai B.J., Hochner B., Mahaut-Smith M., Adams S.R., Kaang B.K., Kandel E.R., Tsien R.Y. 1993. Spatially resolved dynamics of cAMP and protein kinase A subunits in Aplysia sensory neurons. Science. 260:222–226 10.1126/science.7682336 - DOI - PubMed
    1. Barnes A.P., Livera G., Huang P., Sun C., O’Neal W.K., Conti M., Stutts M.J., Milgram S.L. 2005. Phosphodiesterase 4D forms a cAMP diffusion barrier at the apical membrane of the airway epithelium. J. Biol. Chem. 280:7997–8003 10.1074/jbc.M407521200 - DOI - PubMed
    1. Beca S., Helli P.B., Simpson J.A., Zhao D., Farman G.P., Jones P.P., Tian X., Wilson L.S., Ahmad F., Chen S.R., et al. 2011. Phosphodiesterase 4D regulates baseline sarcoplasmic reticulum Ca2+ release and cardiac contractility, independently of L-type Ca2+ current. Circ. Res. 109:1024–1030 10.1161/CIRCRESAHA.111.250464 - DOI - PMC - PubMed
    1. Bender A.T., Beavo J.A. 2006. Cyclic nucleotide phosphodiesterases: molecular regulation to clinical use. Pharmacol. Rev. 58:488–520 10.1124/pr.58.3.5 - DOI - PubMed

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