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. 2013 Oct;16(10):1088-94.

Antibacterial Activity of Elephant Garlic and Its Effect against U2OS Human Osteosarcoma Cells

Zehao Huang  1 , Jianwu Ren  1
Affiliations

Antibacterial Activity of Elephant Garlic and Its Effect against U2OS Human Osteosarcoma Cells

Zehao Huang et al. Iran J Basic Med Sci. 2013 Oct.

Abstract

Objective(s): The present study was designed to investigate the antibacterial function and pharmacological effect of elephant garlic (Allium ampeloprasum var. ampeloprasum) on U2OS human osteosarcoma cells.

Materials and methods: Seven kinds of bacteria were reconstituted, inoculated and tested in this research to evaluate elephant garlic antibacterial activity. By the means of FACS analysis, cell proliferation assay, confocal laser scanning microscopy and Transwell migration assays, the effect of elephant garlic against U2OS human osteosarcoma cells was unveiled. Rerults: The antimicrobial activity of elephant garlic was stronger than ampicillin when used against Escherichia coli, Bacillus subtilis, Bacillus thuringiensis, Staphylococcus actinomycetes, and gray actinomycetes. Even at a very low concentration (12.5%), elephant garlic still had an antibacterial effect on common bacteria E. coli and S. aureus. The G0/G1 ratio of elephant garlic treated group cells increased while S phase decreased. Elephant garlic extract inhibited the growth of human osteosarcoma cells, U2OS, through preventing the transition from G1 phase to S phase. It reduced osteosarcoma cell, U2OS, invasion ability and significantly increased the proportion of apoptosis. It significantly affected the cytoskeleton generation.

Conclusion: Elephant garlic exhibits antibacterial property and has an inhibitory effect on osteosarcoma cells (U2OS) proliferation and cell activity, suggesting the mechanism of its anticancer effects on U2OS human osteosarcoma cells.

Keywords: Anticancer; Bacteriostasis; Elephant garlic; Osteosarcoma.

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Figures

Figure 1
Figure 1
Antibacterial activity of Allium ampeloprasum L on Bacillus thuringiensis during the course of different treatment
Figure 2
Figure 2
Effect of A. ampeloprasum L on viability of U2OS cells (P< 0.001
Figure 3
Figure 3
Effect of A. ampeloprasum L on proliferation of U2OS cells (P< 0.001
Figure 4
Figure 4
Effect of Allium ampeloprasum L on the morphology of U2OS cells
Figure 5
Figure 5
The effect of elephant garlic on apoptosis and necrosis induction in U2OS. High numbers of apoptosis cells are present in the treatment group. The cells were collected after 24 hr of exposure, then stained with FITC and PI (n=4), followed by two-color FACS analysis for PI and FITC. P <0.01
Figure 6
Figure 6
Elephant garlic affects the metastasis of U2OS
See this image and copyright information in PMC

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