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. 2013 Sep;10(3):138-41.
doi: 10.7497/j.issn.2095-3941.2013.03.003.

Identification of differentially expressed long non-coding RNAs in human ovarian cancer cells with different metastatic potentials

Shi-Ping Liu  1 , Jia-Xin Yang  1 , Dong-Yan Cao  1 , Keng Shen  1
Affiliations

Identification of differentially expressed long non-coding RNAs in human ovarian cancer cells with different metastatic potentials

Shi-Ping Liu et al. Cancer Biol Med. 2013 Sep.

Abstract

Objective: To identify differentially expressed long non-coding RNAs (lncRNAs) involved in the metastasis of epithelial ovarian cancer.

Methods: An in vitro invasion assay was performed to validate the invasive capability of SKOV3 and SKOV3.ip1 cell lines. Total RNA was then extracted, and microarray analysis was performed. Moreover, nine lncRNAs were selected for validation using RT-qPCR.

Results: Compared with the SKOV3 cells, the SKOV3.ip1 cells significantly improved in the in vitro invasive activity. Of the 4,956 lncRNAs detected in the microarray, 583 and 578 lncRNAs were upregulated and downregulated, respectively, in SKOV3.ip1 cells, compared with the parental SKOV3 cells. Seven of the analyzed lncRNAs (MALAT1, H19, UCA1, CCAT1, LOC645249, LOC100128881, and LOC100292680) confirmed the deregulation found by microarray analysis.

Conclusion: LncRNAs clusters were differentially expressed in ovarian cancer cells with varying metastatic potentials. This result indicates that some lncRNAs might exert a partial or key role in epithelial ovarian cancer metastasis. Further studies should be conducted to determine the roles of these lncRNAs in ovarian cancer metastasis.

Keywords: Neoplasm metastasis; RNA, long untranslated; ovarian neoplasms.

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Conflict of interest statement

No potential conflicts of interest are disclosed.

Figures

Figure 1
Figure 1
SKOV3.ip1 cells are more invasive than SKOV3 cells (*P<0.01).
See this image and copyright information in PMC

References

    1. Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin 2011;61:69-90 - PubMed
    1. Siegel R, Naishadham D, Jemal A.Cancer statistics, 2013. CA Cancer J Clin 2013;63:11-30 - PubMed
    1. SEER cancer statistics review; 1975-2008. [http://seer.cancer.gov/csr/1975_2008/, based on November 2010 SEER data submission, posted to the SEER web site, 2011.]
    1. Yu G, Yao W, Wang J, Ma X, Xiao W, Li H, et al. LncRNAs expression signatures of renal clear cell carcinoma revealed by microarray. PLoS One 2012;7:e42377. - PMC - PubMed
    1. Yang F, Zhang L, Huo XS, Yuan JH, Xu D, Yuan SX, et al. Long noncoding RNA high expression in hepatocellular carcinoma facilitates tumor growth through enhancer of zeste homolog 2 in humans. Hepatology 2011;54:1679-1689 - PubMed

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