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. 1987 Jun 11;15(11):4403-15.
doi: 10.1093/nar/15.11.4403.

Site-directed cleavage of RNA

S Shibahara, S Mukai, T Nishihara, H Inoue, E Ohtsuka, H Morisawa
Free PMC article

Site-directed cleavage of RNA

S Shibahara et al. Nucleic Acids Res. .
Free PMC article

Abstract

Using complementary chimeric oligonucleotides containing deoxyribonucleotides and 2'-O-methylribonucleotides (1), enzymatically synthesized RNA (90 mer) were cleaved at a single site with Escherichia coli RNaseH, either at a hairpin loop or at a stem region. Especially, site-specific cleavage occurred in even the target region being enclosed within a stable, base-paired stem. The method is proved to be generally applicable to RNA containing secondary structures.

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