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Review
. 2014 Jan 2;14(1):13-26.
doi: 10.1016/j.stem.2013.12.005.

Human pluripotent stem cell culture: considerations for maintenance, expansion, and therapeutics

Kevin G Chen  1 , Barbara S Mallon  2 , Ronald D G McKay  3 , Pamela G Robey  4
Affiliations
Review

Human pluripotent stem cell culture: considerations for maintenance, expansion, and therapeutics

Kevin G Chen et al. Cell Stem Cell. .

Abstract

Human pluripotent stem cells (hPSCs) provide powerful resources for application in regenerative medicine and pharmaceutical development. In the past decade, various methods have been developed for large-scale hPSC culture that rely on combined use of multiple growth components, including media containing various growth factors, extracellular matrices, 3D environmental cues, and modes of multicellular association. In this Protocol Review, we dissect these growth components by comparing cell culture methods and identifying the benefits and pitfalls associated with each one. We further provide criteria, considerations, and suggestions to achieve optimal cell growth for hPSC expansion, differentiation, and use in future therapeutic applications.

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Figures

Figure 1
Figure 1
Timeline of the development of cell culture modules for hPSCs and future directions (Year 2014 to 2016).
See this image and copyright information in PMC

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