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. 1987 Feb 1;241(3):693-7.
doi: 10.1042/bj2410693.

Identification of a phosphoprotein in the nuclear matrix by monoclonal antibodies

M J Halikowski, C C Liew

Identification of a phosphoprotein in the nuclear matrix by monoclonal antibodies

M J Halikowski et al. Biochem J. .

Abstract

Previous work in this laboratory has established that a rat liver nuclear phosphoprotein (B2:Mr 68,000, pI 6.5-8.2) is associated with actively transcribed nucleosomes, as demonstrated by its preferential release after mild treatment with micrococcal nuclease. In the present report we provide further immunological evidence ('Western Blot' analysis, solid-phase radioimmunoassay and indirect immunofluorescence) that in addition establishes the presence of this phosphoprotein in the nuclear-matrix protein fraction. This paradoxical localization suggests that this phosphoprotein may function in two separate and distinct roles within the realm of nuclear organization.

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