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. 2014 Feb;7(2):478-482.
doi: 10.3892/etm.2013.1435. Epub 2013 Dec 4.

Effect of oxidative stress on heme oxygenase-1 expression in patients with gestational diabetes mellitus

Gang Xin  1 , Juan DU  2 , Yong-Tao Wang  3 , Ting-Ting Liang  1
Affiliations

Effect of oxidative stress on heme oxygenase-1 expression in patients with gestational diabetes mellitus

Gang Xin et al. Exp Ther Med. 2014 Feb.

Abstract

The anti-oxidative stress effect of heme oxygenase-1 (HO-1) expression is being increasingly studied. However, few studies regarding HO-1 have been conducted in patients with gestational diabetes mellitus (GDM). In the present study, HO-1 expression was compared in peripheral blood mononuclear cells at 24-28 weeks of pregnancy in patients with GDM and healthy females, to investigate the correlation between HO-1 and oxidative stress by calculation of MDA content in the peripheral blood serum (thiobarbituric acid method), tested ROS (flow cytometry method), HO-1mRNA (RT-PCR method), and HO-1 protein (western blotting method) of Mononuclear cells. The results show that the levels of serum malonaldehyde (MDA), reactive oxygen species (ROS), HO-1 mRNA and HO-1 protein expression in peripheral blood mononuclear cells were higher in the GDM group than in the control group. Correlation analysis showed that the expression levels of HO-1 protein were positively correlated with the HO-1 mRNA expression levels (r=0.680; P=0.000), and the levels of ROS (r=0.572; P=0.000) and MDA (r=0.614; P=0.000). HO-1 mRNA expression levels were found to positively correlate with the levels of MDA (r=0.451; P=0.010) and fasting plasma glucose (FPG; r=0.337; P=0.039). Partial correlation analysis demonstrated that, after removing the effects of body mass index, FPG and 2-h plasma glucose, HO-1 protein expression levels were positively correlated with the levels of HO-1 mRNA expression (r=0.611; P=0.005), ROS (r=0.526; P=0.021) and MDA (r=0.519; P=0.015). These findings indicate that pregnant females with GDM may be protected against oxidative injury due to the induction of adaptive and compensatory expression of HO-1 to guard against oxidative stress induced by high glucose levels.

Keywords: anti-oxidative stress; gestational diabetes mellitus; heme oxygenase-1.

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Figures

Figure 1
Figure 1
Patient selection.
Figure 2
Figure 2
Expression of HO-1 mRNA, as detected by reverse transcription polymerase chain reaction. HO-1 mRNA expression in peripheral blood mononuclear cells from the GDM group was higher than that in peripheral blood mononuclear cells from the control group. GDM, gestational diabetes mellitus; HO-1, heme oxygenase-1.
Figure 3
Figure 3
Western blot analysis of the expression of HO-1 (32 kDa) and the reference β-actin protein in the two groups. The results indicate that HO-1 protein expression in peripheral blood mononuclear cells from the GDM group was higher than that in peripheral blood mononuclear cells from the control group. GDM, gestational diabetes mellitus; HO-1, heme oxygenase-1.
See this image and copyright information in PMC

References

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