Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2014 Jan 9:13:5.
doi: 10.1186/1475-2859-13-5.

Carbon source dependent promoters in yeasts

Katrin Weinhandl, Margit Winkler, Anton Glieder, Andrea Camattari  1
Affiliations
Review

Carbon source dependent promoters in yeasts

Katrin Weinhandl et al. Microb Cell Fact. .

Abstract

Budding yeasts are important expression hosts for the production of recombinant proteins.The choice of the right promoter is a crucial point for efficient gene expression, as most regulations take place at the transcriptional level. A wide and constantly increasing range of inducible, derepressed and constitutive promoters have been applied for gene expression in yeasts in the past; their different behaviours were a reflection of the different needs of individual processes.Within this review we summarize the majority of the large available set of carbon source dependent promoters for protein expression in yeasts, either induced or derepressed by the particular carbon source provided. We examined the most common derepressed promoters for Saccharomyces cerevisiae and other yeasts, and described carbon source inducible promoters and promoters induced by non-sugar carbon sources. A special focus is given to promoters that are activated as soon as glucose is depleted, since such promoters can be very effective and offer an uncomplicated and scalable cultivation procedure.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Target genes for inducible (orange) and derepressed (blue) carbon source dependent promoters in yeasts and their localization in the metabolism.
Figure 2
Figure 2
Mechanism of glucose repression in yeast; modified from [16].
Figure 3
Figure 3
Regulation and function of the GAL genes.
See this image and copyright information in PMC

References

    1. Waterham HR, Digan ME, Koutz PJ, Lair SV, Cregg JM. Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter. Gene. 1997;186:37–44. doi: 10.1016/S0378-1119(96)00675-0. - DOI - PubMed
    1. Tuite M, Dobson MJ, Roberts NA, King RM, Burke DC, Kingsman SM, Kingsman AJ. Regulated high efficiency expression of human interferon-alpha in Saccharomyces cerevisiae. EMBO J. 1982;1(5):603–608. - PMC - PubMed
    1. Gatignol A, Dassain M, Tiraby G. Cloning of Saccharomyces cerevisiae promoters using a probe vector based on phleomycin resistance. Gene. 1990;91(1):35–41. doi: 10.1016/0378-1119(90)90159-O. - DOI - PubMed
    1. Li N, Zhang LM, Zhang KQ, Deng JS, Prändl R, Schöffl F. Effects of heat stress on yeast heat shock factor-promoter binding in vivo. Acta Biochim Biophys Sin. 2006;38(5):356–362. doi: 10.1111/j.1745-7270.2006.00170.x. - DOI - PubMed
    1. Koller A, Valesco J, Subramani S. The CUP1 promoter of Saccharomyces cerevisiae is inducible by copper in Pichia pastoris. Yeast. 2000;16:651–656. doi: 10.1002/(SICI)1097-0061(200005)16:7<651::AID-YEA580>3.0.CO;2-F. - DOI - PubMed

Publication types

MeSH terms