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. 2013 Oct;4(4):421-6.
doi: 10.4103/0976-237X.123015.

A new concept in hybridization: Bromelain enzyme for deproteinizing dentin before application of adhesive system

Raad Niama Dayem  1 , Mona Adnan Tameesh  1
Affiliations

A new concept in hybridization: Bromelain enzyme for deproteinizing dentin before application of adhesive system

Raad Niama Dayem et al. Contemp Clin Dent. 2013 Oct.

Abstract

Objective: The objective of this study is to assess the deproteinizing effect of bromelain enzyme and compare it with neodymium-doped yttrium aluminum garnet (Nd:YAG) laser and 10% sodium hypochlorite (NaOCl) by using scanning electron microscope (SEM) and polarized microscope.

Materials and methods: A total of 60 extracted human upper premolars were selected to be given standardized buccal and lingual class V cavities. The teeth were divided into three groups each one consisted of 20 teeth. Thirty teeth were recruited for SEM study and the other 30 for polarized microscope. Group 1: Teeth were deproteinized with Nd:YAG laser, Group 2: Teeth were deproteinized with bromelain enzyme and Group 3: Teeth were deproteinized with 10% NaOCl.

Results and conclusions: Application of bromelain enzyme has led to removing collagen network and significantly decreased the global leakage scores of the adhesive system.

Keywords: Adhesive system; bromelain enzyme; deproteinizing dentin.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Scanning electron micrograph of the acid-etched dentin surface after treatment with neodymium-doped yttrium aluminum garnet laser
Figure 2
Figure 2
Scanning electron micrograph of the acid-etched dentin surface after treatment with bromelain enzyme
Figure 3
Figure 3
Scanning electron micrograph of the acid-etched dentin surface after treatment with 10% sodium hypochlorite
Figure 4
Figure 4
Mean of the global leakage scores of each treatment
See this image and copyright information in PMC

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