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. 2013 Aug 31;7(2):164-72.
eCollection 2013.

Detection of naturally infected vector ticks (acari: ixodidae) by different species of babesia and theileria agents from three different enzootic parts of iran

Mohammad Abdigoudarzi  1
Affiliations

Detection of naturally infected vector ticks (acari: ixodidae) by different species of babesia and theileria agents from three different enzootic parts of iran

Mohammad Abdigoudarzi. J Arthropod Borne Dis. .

Abstract

Background: Diagnostic study of vector ticks for different pathogens transmitted specifically have been done by Iranian old scientists working on the basis of biological transmission of pathogens. In this study we decided to confirm natural infection of different collected ticks from three different provinces of Iran.

Methods: Ticks were collected from livestock (sheep, goats and cattle) during favorable seasons (April to September 2007 and 2008). Slide preparations were stained by Giemsa and Feulgen and were studied searching for any trace of infection. Positive DNA from infected blood or tissue samples was provided and was used as positive control. First, PCR optimization for positive DNA was done, and then tick samples were subjected to specific PCR.

Results: Eleven pairs of primers were designed for detection of Theileria, Babesia and Anaplasma spp. Totally 21 tick samples were detected to be infected with protozoa. Hyalomma anatolicum anatolicum and Rhipicephalus turanicus from Fars Province were infected with T. lestoquardi at two different places. Hyalomma detritum was infected with T. lestoquardi in Lorestan Province and Rh. turanicus was infected to Ba. ovis from Fars Province.

Conclusion: Totally 21 tick samples were detected to be infected with protozoa. Every sample is regarded with host-environment related factors. Since there are complex relations of vectors and their relevant protozoa, different procedures are presented for future studies.

Keywords: Ixodidae; Molecular Detection; Tick; Vector.

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Figures

Fig. 1 and Fig. 2.
Fig. 1 and Fig. 2.
Wet mount preparation of Salivary glands of suspected ticks, staining was done by Giemsa and Feulgen, clusters are acini of salivary gland.
Fig. 3.
Fig. 3.
Sample photo of Agarose gel electrophoresis of pcr products related to extracted DNA from ticks (14 specimens) (Fars Province) and specific primer for ITS2. (No. 5, 9, 11, 12, 13) are very specific good amplified bands confirming the quality and quantity of DNA
Fig. 4.
Fig. 4.
Agarose gel electrophoresis of pcr products related to DNA positive for T. lestoquardi (line 5) and Babesia ovis (line 7) and specific primers for Slag and Babesia ovis. These (pcr products) (from known positive DNA) have been used as positive control
Fig. 5.
Fig. 5.
Agarose gel electrophoresis of a 500 bp positive band (S) from a suspected tick (group 5 of Shiraz), Primers are (Tams1, Tams4) followed by (Tams3, Tams2) (a nested pcr was applied) and the infection of tick with T. annulata was confirmed. (Marker is 100 ladder marker) (S= sample)
Fig. 6.
Fig. 6.
Agarose gel electrophoresis of pcr products related to DNA group no. 4 (19 specimens) and specific primers for Babesia ovis (Bab3, 4). No. 5 (500 bp), 16, 18, 19 and 20 regarded positive and related individual ticks were recorded infected to Babesia ovis. (M= 100bp Marker)
See this image and copyright information in PMC

References

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