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Comparative Study
. 2014 Mar;33(3):182-8.
doi: 10.1089/dna.2013.2277. Epub 2014 Jan 11.

Distribution and clonality of the vα and vβ T-cell receptor repertoire of regulatory T cells in leukemia patients with and without graft versus host disease

Zhenyi Jin  1 , Xiuli Wu, Shaohua Chen, Lijian Yang, Qifa Liu, Yangqiu Li
Affiliations
Comparative Study

Distribution and clonality of the vα and vβ T-cell receptor repertoire of regulatory T cells in leukemia patients with and without graft versus host disease

Zhenyi Jin et al. DNA Cell Biol. 2014 Mar.

Abstract

Graft versus host disease (GVHD) is the main complication following allogeneic hematopoietic stem cell transplantation (allo-HSCT). Recent data indicated that regulatory T (Treg) cells might relate to GVHD, and such functions might be mediated by certain T-cell receptor (TCR) subfamily of Treg cells. Thus, we analyzed the distribution and clonality of the TCR Vα and Vβ repertoire of Treg cells from leukemia patients with and without GVHD after allo-HSCT. Numerous TCR Vα subfamilies, including Vα1, Vα9, Vα13, Vα16-19, and Vα24-29, were absent in Treg cells after allo-HSCT. The usage numbers for the TCR Vα and Vβ subfamilies in Treg cells from patients without GVHD appeared more widely. The expression frequencies of Vα10 or Vα20 between both groups were significantly different. Moreover, the expression frequency of TCR Vβ2 subfamily in patients without GVHD was significantly higher than that in patients with GVHD. Oligoclonally expanded TCR Vα and Vβ Treg cells were identified in a few samples in both groups. Restricted utilization of the Vα and Vβ subfamilies and the absence of some important TCR rearrangements in Treg cells may be related to GVHD due to a lower regulating function of Treg subfamilies.

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Figures

<b>FIG. 1.</b>
FIG. 1.
The expression frequencies of T-cell receptor (TCR) Vα (A) and Vβ (B) subfamilies in Treg cells from patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT) with and without graft versus host disease (GVHD). *Compared the expression frequency of TCR Vα or Vβ subfamilies of Treg cells in patients with GVHD onset and without GVHD.
<b>FIG. 2.</b>
FIG. 2.
The distribution and clonality of TCR Vα (A) and Vβ (B) subfamilies in Treg cells from patients after allo-HSCT. Patients with GVHD: numbered G01 to G22; Patients without GVHD: numbered NG01 to NG08.
<b>FIG. 3.</b>
FIG. 3.
Clonality differences of the TCR Vα and Vβ subfamilies in Treg cells between patients with and without GVHD. (A) Oligoclonal expansion of the TCR Vα and Vβ subfamilies in Treg cells from patients with GVHD. (B) Polyclonal expansion of the TCR Vα and Vβ subfamilies in Treg cells from patients without GVHD. (C) Oligoclonal expansion of the TCR Vα and Vβ subfamilies in Treg cells from patients without GVHD. (D) Polyclonal expansion of the TCR Vα and Vβ subfamilies in Treg cells from patients with GVHD.
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