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. 1991 Jun;28(3):149-53.
doi: 10.1007/BF00054128.

Rapid and simple isolation of pure photosystem II core and reaction center particles from spinach

P J van Leeuwen  1 , M C Nieveen, E J van de Meent, J P Dekker, H J van Gorkom
Affiliations

Rapid and simple isolation of pure photosystem II core and reaction center particles from spinach

P J van Leeuwen et al. Photosynth Res. 1991 Jun.

Abstract

Pure and active oxygen-evolving PS II core particles containing 35 Chl per reaction center were isolated with 75% yield from spinach PS II membrane fragments by incubation with n-dodecyl-β-D-maltoside and a rapid one step anion-exchange separation. By Triton X-100 treatment on the column these particles could be converted with 55% yield to pure and active PS II reaction center particles, which contained 6 Chl per reaction center.

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References

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