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. 1996 May;12(3):297-9.
doi: 10.1007/BF00360933.

Effect of carbon source on the β-glucosidase system of the thermophilic fungus Humicola grisea

M L Polizeli  1 , J A Jorge, H F Terenzi
Affiliations

Effect of carbon source on the β-glucosidase system of the thermophilic fungus Humicola grisea

M L Polizeli et al. World J Microbiol Biotechnol. 1996 May.

Abstract

H. grisea produced an extracellular β-glucosidase (EC 3.2.1.21) at high activity in media supplemented with carboxymethyl cellulose (CMC) or cellobiose. Cellobiose-induced β-glucosidase was insensitive to glucose repression whereas that of CMC-supplemented cultures was partially repressed. Molecular sieving revealed three main active components (Mr 50, 128 and 240 kDa). Glucose competitively inhibited β-glucosidase activities with Ki values of 0.9MM and 3.3MM (extracellular) and 10.2MM and 22.6MM (cytosolic), induced in the presence of CMC or cellobiose respectively.

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