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. 1993 Sep;9(5):574-8.
doi: 10.1007/BF00386297.

Partial purification and properties of pectin lyase from Penicillium expansum

D O Silva  1 , M M Attwood, D W Tempest
Affiliations

Partial purification and properties of pectin lyase from Penicillium expansum

D O Silva et al. World J Microbiol Biotechnol. 1993 Sep.

Abstract

A pectin lyase, poly(methoxygalacturonide) lyase, EC 4.2.2.10, from a culture filtrate of Penicillium expansum was partially purified 33-fold with 7.3% yield. The enzyme was monomeric with a molecular mass of 36.5 kDa. The enzyme did not contain pectate lyase activity and degraded citrus and apple pectin best at pH 7.0 and 40 to 45°C. The K m for citrus pectin was 9 mg ml(-1).

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