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. 1994 Jan;10(1):33-5.
doi: 10.1007/BF00357559.

Production of a thermophilic, extracellular alkaline protease by Bacillus stearothermophilus AP-4

R Dhandapani  1 , R Vijayaragavan
Affiliations

Production of a thermophilic, extracellular alkaline protease by Bacillus stearothermophilus AP-4

R Dhandapani et al. World J Microbiol Biotechnol. 1994 Jan.

Abstract

A thermophilic Bacillus stearothermophilus strain AP-4 excreting a thermostable alkaline protease, was isolated from a local compost. Maximum activity of protease (250 U/ml) was after 36 h growth in broth at pH 9.0 and at 55°C. The protease was optimally active at pH 9.0 and 55°C and was stable in 5 mM CaCl2. The enzyme was completely inactivated by PMSF, EDTA and β-mercaptoethanol. It is therefore a metal ion-dependent, alkaline, serine protease.

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