Detection of heat-stable enterotoxigenic Escherichia coli by hybridization with an RNA transcript probe
- PMID: 2442192
- PMCID: PMC269277
- DOI: 10.1128/jcm.25.8.1572-1573.1987
Detection of heat-stable enterotoxigenic Escherichia coli by hybridization with an RNA transcript probe
Abstract
Heat-stable enterotoxigenic Escherichia coli was identified by nucleotide hybridization with RNA transcripts of the gene encoding heat-stable A-2 enterotoxin. Radiolabeled enterotoxin gene RNA transcripts are easier to prepare and avoid the preparation of cloned DNA probes that can be nonspecific if they contain cloning vector DNA.
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