Study of expression of TLR2, TLR4 and transckription factor NF-kB structures of galt of rats in the conditions of the chronic social stress and modulation of structure of intestinal microflora

Abstract

The present study was conducted to investigate of the influence of chronic social stress (CSS) and modulation of the composition of intestinal microflora on the distribution of TLR2+-, TLR4+- and Nf-kB+-cells in the GALT of ileum of the rats. Researchers have been conducted on 84 rats (female) of Wistar line, which were divided on 7 experimental groups: control rats (group 1); rats, which were modeled CSS1 by means of three weeks social isolation and prolong psychoemotional influence (group2); rats, which having CSS 2 modeling by means of keeping animals in over populated cages with every day change of grouping (group 3); rats with CSS1 and CSS2, which were made the modeling of intestinal microflora by means of administrations of aminoglycosed antibiotic kanamycin (group 4 and 5, accordingly); rats with CSS1 and CSS2, which were made the modeling of intestinal microflora by means of everyday administrations of lactobacterine (groups 6 and 7, accordingly). Structure of population of TLR2+-, TLR4+- and Nf-kB+-cells has been studied by the analysis of serial histological sections using the method of direct and indirect immunofluorescense with monoclonal antibodies to TLR2, TLR4 and Nf-kB. CSS development is accompanied with increase in total lymphocytes expressing TLR2 and 4 type GALT rats with the most pronounced in LFV (TLR2+-lymphocytes) and PP LFs (TLR4+-cells) led to an increase in the number of Nf-kB+- cells: in LFV a 1.8-2 fold (p<0.05) in PP at the sub - 52-91% (p<0.05) in PP LFs - for 89-92% (p<0.05), and it is also influenced on the density of TLR2, TLR4, and the concentration of Nf-kB in immunopositive cells. AB and PB injections were accompanied by a decrease in the number of studied cells, so in the separate zone GALT is increased to their number, changing the density of immune system receptors.