Recovery from photoinhibition: effect of light and inhibition of protein synthesis of 32-kD chloroplast protein

Abstract

Recovery from photoinhibition of photosynthesis in intact Lemna gibba was studied in presence of the protein synthesis inhibitors chloramphenicol and cycloheximide. Exposure to an irradiance of 1000 μmol m(-2)s(-1) in N2 for 90 min induced 80% photoinhibition. The plants recovered photosynthesis when transfered to normal irradiances (210 μmol m(-2)s(-1)) and air. Chloramphenicol added to the medium was taken up by the plant and reduced photosynthesis slightly. Recovery from photoinhibition was more inhibited than photosynthesis. Cycloheximide was also taken up by the plants and reduced synthesis of light harvesting chlorophyll protein: however, neither photosynthesis nor recovery were much affected. Synthesis of 32-kD chloroplast protein during recovery was inhibited by chloramphenicol, but not by cycloheximide. Synthesis of 32-kD protein was enhanced by 20-210 μmol m(-2)s(-1) light. The results support the hypothesis that synthesis of 32-kD protein is important for recovery of photosynthesis after photoinhibition.