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. 1991 Jan;7(1):12-6.
doi: 10.1007/BF02310912.

Characteristics of a highly thermostable neutral protease produced fromBacillus stearothermophilus

Y Fujio  1 , S Kume
Affiliations

Characteristics of a highly thermostable neutral protease produced fromBacillus stearothermophilus

Y Fujio et al. World J Microbiol Biotechnol. 1991 Jan.

Abstract

A highly thermostable neutral protease was found in culture filtrates ofBacillus stearothermophilus. The optimum reaction pH and temperature of this protease were 6.0 and 60°C, respectively, and 90% activity remained even after heat treatment at 90°C for 30 min. The protease was markedly inactivated by diisopropyl fluorophosphate, but EDTA and iodoacetic acid hardly affected it. The neutral protease therefore could be defined as a highly thermostable, neutral(-serine) protease.

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References

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