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. 1977 Jan;133(3):215-7.
doi: 10.1007/BF00380679.

Purification and identification of a cytokinin from moss callus cells

P Beutelmann  1 , L Bauer
Affiliations

Purification and identification of a cytokinin from moss callus cells

P Beutelmann et al. Planta. 1977 Jan.

Abstract

A cytokinin was isolated from the culture medium of callus cells of the moss hybridFunaria hygrometrica (L.) Sibth xPhyscomitrium piriforme Brid. The purification procedure included ethyl-acetate extraction, silver-salt precipitation, crystallization as picrate, and ion exchange chromatography. The structure of the cytokinin was confirmed as N(6)-(Δ(2)-isopentenyl)adenine by means of gas chromatography and mass spectrometry. The concentration of the compound in the culture medium was determined at ca. 10(-6) M.

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