Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Jan;133(3):267-73.
doi: 10.1007/BF00380688.

Coumarin inhibition of microfibril formation at the surface of cultured protoplasts

J Burgess  1 , P J Linstead
Affiliations

Coumarin inhibition of microfibril formation at the surface of cultured protoplasts

J Burgess et al. Planta. 1977 Jan.

Abstract

The effect of including coumarin in the culture medium of isolated tobacco leaf protoplasts has been studied by scanning microscopy. Fibre formation is completely suppressed for 48 h in concentrations of 200 and 250 mg/l of coumarin. Continuous culture in these concentrations of coumarin does not result in cell division or colony formation, although an atypical wall is visible after 4 days. The effect of coumarin is reversible. Viability of protoplasts exposed for 48 h to 200 mg/l coumarin is unaffected in terms of plating efficiency and infectability with virus. These results are discussed in relation to cell wall synthesis and the possible use of coumarin in prolonging the protoplast state.

PubMed Disclaimer

References

    1. Planta. 1976 Jan;130(1):73-9 - PubMed
    1. Planta. 1976 Jan;131(2):173-8 - PubMed
    1. Planta. 1975 Jan;127(3):281-4 - PubMed
    1. Planta. 1971 Mar;99(1):12-20 - PubMed
    1. J Gen Virol. 1973 Aug;20(2):177-93 - PubMed