Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Jan;133(3):303-8.
doi: 10.1007/BF00380693.

β-Glucan synthetase activity in Golgi vesicles ofPetunia hybrida

J P Helsper  1 , J H Veerkamp, M M Sassen
Affiliations

β-Glucan synthetase activity in Golgi vesicles ofPetunia hybrida

J P Helsper et al. Planta. 1977 Jan.

Abstract

β-Glucan synthetase activity has been demonstrated in a Golgi vesicle fraction isolated from pollen tubes ofPetunia hybrida. Thisβ-glucan synthetase activity differs from that of most other higher plants in its inability to incorporate [(14)C]glucose from GDP-[(14)C]glucose. UDP-[(14)C]glucose, however, is an appropriate glucose donor for this enzyme. The optimum conditions for thisβ-glucan synthetase activity are: 1 mg Golgi vesicle protein/ml reaction mixture; pH=±8 and a temperature of 25°C. The newly synthesized alkali-insoluble glucan containsβ-1,3- as well as β-1,4-glucosidic linkages.

PubMed Disclaimer

References

    1. Biochim Biophys Acta. 1975 Jan 13;381(1):97-108 - PubMed
    1. FEBS Lett. 1970 Jun 8;8(4):224-228 - PubMed
    1. Proc Natl Acad Sci U S A. 1969 Oct;64(2):605-12 - PubMed
    1. Plant Physiol. 1975 Jul;56(1):83-7 - PubMed
    1. J Biol Chem. 1958 Jun;232(2):627-36 - PubMed