Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2014 Jan 2:4:299.
doi: 10.3389/fgene.2013.00299.

The CAF1-NOT complex of trypanosomes

Esteban Erben  1 , Chaitali Chakraborty  1 , Christine Clayton  1
Affiliations
Review

The CAF1-NOT complex of trypanosomes

Esteban Erben et al. Front Genet. .

Abstract

In African trypanosomes, there is no control of transcription initiation by RNA polymerase II at the level of individual protein-coding genes. Transcription is polycistronic, and individual mRNAs are excised by trans-splicing and polyadenylation. As a consequence, trypanosomes are uniquely reliant on post-transcriptional mechanisms for control of gene expression. Rates of mRNA decay vary over up to two orders of magnitude, making these organisms an excellent model system for the study of mRNA degradation processes. The trypanosome CAF1-NOT complex is simpler than that of other organisms, with no CCR4 or NOT4 homolog: it consists of CAF1, NOT1, NOT2, NOT5 NOT9, NOT10, and NOT11. It is important for the initiation of degradation of most, although not all, mRNAs. There is no homolog of NOT4, and Tho and TREX complexes are absent. Functions of the trypanosome NOT complex are therefore likely to be restricted mainly to deadenylation. Mechanisms that cause the NOT complex to deadenylate some mRNAs faster than others must exist, but have not yet been described.

Keywords: CAF1; NOT complex; Trypanosoma; deadenylation; mRNA decay; mRNA degradation.

PubMed Disclaimer

Figures

FIGURE 1
FIGURE 1
Putative subunits of the trypanosome NOT complex, as judged by tandem affinity purification. Association of DHH1 is unclear. Two-hybrid interactions are indicated by arrows.
See this image and copyright information in PMC

References

    1. Barnes R., Shi H., Kolev N., Tschudi C., Ullu E. (2012). Comparative genomics reveals two novel RNAi factors in Trypanosoma brucei and provides insight into the core machinery. PLoS Pathog. 8:e100267810.1371/journal.ppat.1002678 - DOI - PMC - PubMed
    1. Barrett M. P., Croft S. L. (2012). Management of trypanosomiasis and leishmaniasis. Br. Med. Bull. 104 175–19610.1093/bmb/lds031 - DOI - PMC - PubMed
    1. Bawankar P., Loh B., Wohlbold L., Schmidt S., Izaurralde E. (2013). NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT complex that docks onto the NOT1 N-terminal domain. RNA Biol. 10 228–24410.4161/rna.23018 - DOI - PMC - PubMed
    1. Bringaud F., Riviere L., Coustou V. (2006). Energy metabolism of trypanosomatids: adaptation to available carbon sources. Mol. Biochem. Parasitol. 149 1–910.1016/j.molbiopara.2006.03.017 - DOI - PubMed
    1. Burki F., Shalchian-Tabrizi K., Pawlowski J. (2008). Phylogenomics reveals a new ‘megagroup’ including most photosynthetic eukaryotes. Biol. Lett. 4 366–36910.1098/rsbl.2008.0224 - DOI - PMC - PubMed