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. 2013 Oct;19(4):529-36.
doi: 10.1007/s12298-013-0184-4.

Salicylic acid and salicylic acid sensitive and insensitive catalases in different genotypes of chickpea against Fusarium oxysporum f. sp. ciceri

S Gayatridevi  1 , S K Jayalakshmi  2 , V H Mulimani  1 , K Sreeramulu  1
Affiliations

Salicylic acid and salicylic acid sensitive and insensitive catalases in different genotypes of chickpea against Fusarium oxysporum f. sp. ciceri

S Gayatridevi et al. Physiol Mol Biol Plants. 2013 Oct.

Abstract

Differential expression of catalase isozymes in different genotypes of chickpea resistant genotypes- A1, JG-315, JG-11, WR-315, R1-315, Vijaya, ICCV-15017, GBS-964, GBM-10, and susceptible genotypes- JG-62, MNK, ICCV-08321, ICCV-08311, KW-104, ICCV-08123, ICC-4951, ICC-11322, ICC-08116 for wilt disease caused by Fusarium oxysporum. f. sp. ciceri (Foc) was analyzed. Salicylic acid (SA) and H2O2 concentrations were determined in control as well as in plants infected with F. ciceri and found that the high and low levels of salicylic acid and H2O2 in resistant and susceptible genotypes of chickpea respectively. Catalase isozyme activities were detected in the gel and found that no induction of new catalases was observed in all the resistant genotypes and their some of the native catalase isozymes were inhibited; whereas, induction of multiple catalase isozymes was observed in all the screened susceptible genotypes and their activities were not inhibited upon Foc or SA treatments. The above results support the possible role of these isozymes as a marker to identify which genotype of chickpea is expressing systemic acquired resistance.

Keywords: Catalase; Chickpea; Fusarium oxysporum f. sp. ciceri; H2O2; Salicylic acid.

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Figures

Fig. 1
Fig. 1
Native gel stained for catalase activity from root and shoot extract of chickpea resistant genotypes JG-315 (1), JG-11(2), A-1 (3) and WR-315 (4), (A) control root; (B) control shoot: (C) SA treated root; (D) SA treated shoot; (E) pathogen infected root; (F) pathogen infected shoot; Equal amount of protein was loaded on gels (20 μg)
Fig. 2
Fig. 2
Native gel stained for catalase activity from root and shoot extract of chickpea susceptible genotypes: JG-62 (1), ICCV-08321 (2), ICCV-08311 (3) and MNK (4). (A) control root; (B) control shoot: (C) SA treated root; (D) SA treated shoot; (E) pathogen infected root; (F) pathogen infected shoot; Equal amount of protein was loaded on gels (20 μg). Arrows indicate induction of new isofoms of catalases
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