Comparison of the acute phase response of cultured Morris hepatoma 7777 cells and of rat hepatocytes

Abstract

Isolated Morris hepatoma cells (line 7777) or adult rat hepatocytes were cultured for 3 days and daily production of four plasma proteins was estimated in the cell media by rocket immunoelectrophoresis with monospecific antisera. Addition of cytokines from rat peritoneal macrophages to cultured hepatocytes or hepatoma cells augmented accumulation in the medium of two positive acute phase proteins: fibrinogen (FIB) and cysteine proteinase inhibitor (CPI). At the same time synthesis of alpha-fetoprotein (AFP) was inhibited in hepatoma cells but remained undetectable in hepatocytes. Rat macrophage cytokines typically depressed synthesis of albumin (ALB) in cultured rat hepatocytes but increased production of this protein by hepatoma cells.