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. 1987 Oct;172(2):481-6.
doi: 10.1016/0014-4827(87)90406-x.

Time dependence of X gene reactivation induced by 5-azacytidine: possible progressive restructuring of chromatin

N Homman  1 , S Heuertz, M C Hors-Cayla
Affiliations

Time dependence of X gene reactivation induced by 5-azacytidine: possible progressive restructuring of chromatin

N Homman et al. Exp Cell Res. 1987 Oct.

Abstract

According to the theoretical mechanism of DNA demethylation by 5-azacytidine, the complete demethylation of one site will require two cell divisions. If reexpression is directly related to demethylation, a maximal reexpression is expected after two cell divisions. In a hamster X human hybrid cell line containing an inactive human X chromosome treated by 5-azacytidine, we show that HPRT reactivation frequency is increased more than 10-fold when cells are allowed to divide 14 times before the selection for the HPRT reactivants. We suggest that the delay corresponds to changes in chromatin conformation.

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