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. 1977 Dec 15;168(3):441-5.
doi: 10.1042/bj1680441.

Demonstration of the phosphorylation of acetyl-coenzyme A carboxylase within intact rat epididymal fat-cells

R W Brownsey, W A Hughes, R M Denton

Demonstration of the phosphorylation of acetyl-coenzyme A carboxylase within intact rat epididymal fat-cells

R W Brownsey et al. Biochem J. .

Abstract

Intact rat epididymal fat-cells were incubated with 32Pi and the intracellular proteins separated by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. One of the phosphorylated proteins has the same RF value as [14C]biotin-labelled acetyl-CoA carboxylase purified from fat-cells and is specifically precipitated after incubation with antiserum raised against acetyl-CoA carboxylase. No significant changes in the extent of phosphorylation of acetyl-CoA carboxylase were detected after exposure of the cells to insulin.

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