Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Oct;84(19):6825-9.
doi: 10.1073/pnas.84.19.6825.

Heterologous enzyme function in Escherichia coli and the selection of genes encoding the catalytic RNA subunit of RNase P

N P Lawrence  1 , A Richman, R Amini, S Altman
Affiliations

Heterologous enzyme function in Escherichia coli and the selection of genes encoding the catalytic RNA subunit of RNase P

N P Lawrence et al. Proc Natl Acad Sci U S A. 1987 Oct.

Abstract

The gene for the catalytic RNA subunit of RNase P has been isolated from several Enterobacteriaceae by complementation of an Escherichia coli strain that is temperature-sensitive for RNase P activity. The selection procedure relies on the ability of the heterologous gene products to function enzymatically in E. coli. This procedure obviates the need for positive results in DNA blot hybridization experiments or for the purification of holoenzyme to identify the RNA component of RNase P and its corresponding gene from organisms other than E. coli. Comparisons of the variations in sequences provide the basis for a refined two-dimensional model of the secondary structure of M1 RNA.

PubMed Disclaimer

References

    1. J Mol Biol. 1975 Dec 15;99(3):419-43 - PubMed
    1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
    1. Annu Rev Genet. 1979;13:393-415 - PubMed
    1. Methods Enzymol. 1980;65(1):75-85 - PubMed
    1. Science. 1981 Dec 11;214(4526):1205-10 - PubMed

Publication types

LinkOut - more resources