[Transport, distribution and metabolism of auxin in Vicia faba L. roots after application of [(14)C]IAA or [ (3)H]IAA to the apical bud]

Abstract

After application of [2-(14)C]IAA or [(3)H]IAA to the apical bud of intact young broad-beans, the movement of labelled auxin into the roots was followed by liquid scintillation counting and by autoradiographic analyses. Its metabolism was studied by chromatography, and its pathways by autoradiographic analyses coupled with ringing experiments or removal of the stele.The movement of [(14)C]IAA or [(3)H]IAA was characterized by a high retention of radioactivity in tissues, particularly in very young plants. The speed, which did not exceed 9 mm·h(-1) in old roots, appeared the slower the younger the plants were. However, it seemed possible that small quantities of IAA or its derivates went into sieve tubes in which they moved downwards faster. In the apical part of the root the labelled IAA was more quickly transformed than in the other parts of this organ. 24 h after the application of the IAA, the labelled molecules gathered more densely in the cap itself than in apical meristem.At least 2/3 of the applied auxin moved within the stele, which in a crosssection represents only 1/7 of the whole area. In the older part of the root, the cambial zone located between mature phloem and mature xylem was the preferred pathway of IAA transport, although it is a zone where the hormone is immobilized, used and metabolised. In the younger part of the root, the whole stele was the preferred pathway. Therefore, the auxin is in a privileged situation to take part in the regulation of various processes, especially in the development of secondary vascular tissue, more particularly of xylem.