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Comment
. 2014 Jan;26(1):2.
doi: 10.1105/tpc.114.122606. Epub 2014 Jan 17.

Geminivirus vectors deliver reagents for plant genome engineering

Jennifer Mach  1
Affiliations
Comment

Geminivirus vectors deliver reagents for plant genome engineering

Jennifer Mach. Plant Cell. 2014 Jan.
No abstract available

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Figures

None
Repair of a transgene and regeneration of callus and plantlets. Left, top: Original transgene containing an operon with a deletion (gray line) in GUS (β-glucuronidase) and NPTII (neomycin phosphotransferase), with GUS in blue and NPTII in red. Left, bottom: Repaired transgene with complete GUS:NPTII sequences and sequence traces from PCR products from regenerated calli. Right: Callus and plantlet stained for GUS activity, in blue. (Reprinted from Baltes et al. [2014], Figure 5.)
See this image and copyright information in PMC

Comment on

  • DNA replicons for plant genome engineering.
    Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF. Baltes NJ, et al. Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792. Epub 2014 Jan 17. Plant Cell. 2014. PMID: 24443519 Free PMC article.

References

    1. Baltes N.J., Gil-Humanes J., Cermak T., Atkins P.A., Voytas D.F. (2014). DNA replicons for plant genome engineering. Plant Cell 26: 151–163 - PMC - PubMed
    1. Belhaj K., Chaparro-Garcia A., Kamoun S., Nekrasov V. (2013). Plant genome editing made easy: Targeted mutagenesis in model and crop plants using the CRISPR/Cas system. Plant Methods 9: 39. - PMC - PubMed