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. 1987 Jul;133(7):1969-74.
doi: 10.1099/00221287-133-7-1969.

Oligonucleotide probes complementary to variable regions of ribosomal RNA discriminate between Mycoplasma species

U B Göbel  1 , A Geiser, E J Stanbridge
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Oligonucleotide probes complementary to variable regions of ribosomal RNA discriminate between Mycoplasma species

U B Göbel et al. J Gen Microbiol. 1987 Jul.

Abstract

On the basis of information from computer-assisted sequence comparison of the Mycoplasma pneumoniae 16S ribosomal RNA (rRNA) sequences with sequences from various other mycoplasmal and bacterial species, we constructed M. pneumoniae-specific oligonucleotide probes complementary to variable regions in the 16S rRNA molecule. Using a DNA/RNA dot blot hybridization procedure, it was possible to detect less than 1 X 10(3) mycoplasmas. This test is a most sensitive assay for species-specific detection of bacteria. It can easily be adapted for detection and identification of other bacterial species and may have wide medical and industrial application.

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