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. 2014 Apr;52(4):1232-4.
doi: 10.1128/JCM.03453-13. Epub 2014 Jan 22.

Comparative assessment of automated nucleic acid sample extraction equipment for biothreat agents

Warren Vincent Kalina  1 , Christina Elizabeth Douglas, Susan Rajnik Coyne, Timothy Devin Minogue
Affiliations

Comparative assessment of automated nucleic acid sample extraction equipment for biothreat agents

Warren Vincent Kalina et al. J Clin Microbiol. 2014 Apr.

Abstract

Magnetic beads offer superior impurity removal and nucleic acid selection over older extraction methods. The performances of nucleic acid extraction of biothreat agents in blood or buffer by easyMAG, MagNA Pure, EZ1 Advanced XL, and Nordiag Arrow were evaluated. All instruments showed excellent performance in blood; however, the easyMAG had the best precision and versatility.

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Figures

FIG 1
FIG 1
Percentage of extracted agent dilutions (1 × 105 to 7.8 × 102 CFU or PFU/ml) that were statistically different between blood and buffer for each instrument. The Arrow is not shown because there was no data for buffer to make comparisons. With the exception of the EZ1 for VEE virus, the easyMAG had similar but overall fewer instances of significantly different Cq values between blood and buffer samples.
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