doi: 10.1016/0003-2697(87)90378-2.
Detection of a few picograms of DNA on polyacrylamide gels by silver staining
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- PMID: 2445219
- DOI: 10.1016/0003-2697(87)90378-2
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Detection of a few picograms of DNA on polyacrylamide gels by silver staining
Anal Biochem.
1987 Jul.
Abstract
DNA fragments separated on polyacrylamide gels are silver stained in ethanolamine solution. The staining procedure can be completed in 3 1/2 h. Illumination of the gels on a black background increases the sensitivity of detection compared with the usual transillumination. The limit level of detection is 3-5 pg per band with a cross-sectional area of 5 mm2. Five to fifty picograms of DNA may be detected quantitatively by scanning the gels. The method will detect 0.1 to 1 ng per band of low-molecular-weight RNA components.
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