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. 1987 Sep;53(9):2013-20.
doi: 10.1128/aem.53.9.2013-2020.1987.

Development of a defined medium and two-step culturing method for improved exotoxin A yields from Pseudomonas aeruginosa

I I Blumentals  1 , R M Kelly, M Gorziglia, J B Kaufman, J Shiloach
Affiliations

Development of a defined medium and two-step culturing method for improved exotoxin A yields from Pseudomonas aeruginosa

I I Blumentals et al. Appl Environ Microbiol. 1987 Sep.

Abstract

A two-step method is described for the production of exotoxin A by Pseudomonas aeruginosa in which a defined growth medium is modified for the toxin production phase. As a result, specific exotoxin A yields comparable to those obtained with complex media were achieved. In the development of this two-step process, several divalent metallic cations (Ca2+, Cu2+, and Mn2+), in addition to iron, were found to inhibit the yield of exotoxin A while Ca2+ and glycerol were found to increase yields. Northern blot analysis of total RNA isolates suggests that these effects on exotoxin A yields are based on events at the transcription level.

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