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. 2013 Dec;5(6):108-12.
Epub 2013 Dec 26.

Sterilizing Endodontic Files by four different sterilization methods to prevent cross-infection - An In-vitro Study

T B V G Raju  1 , Satish Garapati  2 , Rupika Agrawal  3 , Sridhara Reddy  4 , Ankur Razdan  5 , S Kishore Kumar  6
Affiliations

Sterilizing Endodontic Files by four different sterilization methods to prevent cross-infection - An In-vitro Study

T B V G Raju et al. J Int Oral Health. 2013 Dec.

Abstract

Background: Aim of the study was to compare 4 different methods of sterilizing endodontic files in dental practice.

Materials & methods: The present study was performed on 100 K-files, 21 mm long and of size 25. Of these, 20 files were taken as control group, and the remaining 80 files were divided into 4 groups of 20 files each and they were tested for the efficacy of sterilization with different methods: Autoclave, glass bead, glutaraldehyde and CO2 laser.

Results: The study showed that the files sterilized by autoclave and lasers were completely sterile. Those sterilized by glass bead were 90% sterile and those with glutaraldehyde were 80% sterile.

Conclusion: The study concluded that autoclave or laser could be used as a method of sterilization in clinical practice and in advanced clinics; laser can be used also as a chair side method of sterilization. How to cite this article: Raju TB, Garapati S, Agrawal R, Reddy S, Razdan A, Kumar SK. Sterilizing Endodontic Files by four different sterilization methods to prevent cross-infection - An In-vitro Study. J Int Oral Health 2013; 5(6):108-12 .

Keywords: Bacillus Stearothermophillus; Thioglycollate medium; endodontic files; endodontic instrument box; sterilization.

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Conflict of interest statement

Conflict of Interest: None Declared

Figures

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Figure 1: Test tubes after autoclave sterilization
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Figure 3: Test tubes after glutaraldehyde sterilization
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Figure 2: Test tubes after glass-bead sterilization
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Figure 4: Test tubes after laser sterilization
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Graph 1: Graph of comparison of groups
See this image and copyright information in PMC

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