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Comparative Study
. 1987 Nov;33(11):2019-23.

Specific immunoradiometric assay of insulin-like growth factor I with use of monoclonal antibodies

M G Scott  1 , G C Cuca, J R Petersen, L R Lyle, B D Burleigh, W H Daughaday
Affiliations
  • PMID: 2445506
Comparative Study

Specific immunoradiometric assay of insulin-like growth factor I with use of monoclonal antibodies

M G Scott et al. Clin Chem. 1987 Nov.

Abstract

We identified two monoclonal antibodies that bind spatially distinct epitopes on insulin-like growth factor I (IGF-I). Using these two antibodies, we developed a simultaneous, two-site immunoradiometric assay (IRMA) specific for IGF-I. This IRMA has no detectable cross reactivity with insulin, proinsulin, prolactin, or somatotropin, and less than 2% crossreactivity with IGF-II. The assay response varies linearly with IGF-I concentrations of 0-800 micrograms/L in serum; the detection limit is about 10 micrograms/L. A comparison of 26 IGF-I serum values from the IRMA and from a previously reported IGF-I specific RIA gave a correlation coefficient of 0.96 with no substantial bias (slope = 1.10). IGF-I values for serum, as an aid in assessing growth abnormalities, are easily (only three pipetting steps) obtained in less than 4 h.

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