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. 2013 Oct 1;5(4):e187-91.
doi: 10.4317/jced.51121.

Toluidine blue staining as an adjunctive tool for early diagnosis of dysplastic changes in the oral mucosa

Shambulingappa Pallagatti  1 , Soheyl Sheikh  1 , Amit Aggarwal  2 , Deepak Gupta  2 , Ravinder Singh  2 , Roopika Handa  2 , Simranpreet Kaur  3 , Jyoti Mago  3
Affiliations

Toluidine blue staining as an adjunctive tool for early diagnosis of dysplastic changes in the oral mucosa

Shambulingappa Pallagatti et al. J Clin Exp Dent. .

Abstract

Prognosis of oropharyngeal squamous cell carcinoma depends on early diagnosis, despite advanced surgical techniques, the 5-year survival rate remains ~40-50%. Unfortunately, it is usually detected when it becomes symptomatic. This requires treatment which gives rise to a high rate of morbidity and mortality and, furthermore, early detection of oro-pharyngeal pre-malignant lesions is important to improve the survival rate and quality of life. Since dysplasia and in situ carcinoma contain much more DNA and RNA than the normal surrounding epithelium, the use of in vivo staining, by means of toluidine blue dye, is based on the fact that it is an acidophilic dye that selectively stains acidic tissue components such as DNA and RNA. Toluidine blue staining is considered to be sensitive in identifying early oro-pharyngeal premalignant and malignant lesions. In the present study, the use of toluidine blue staining was taken into consideration to identify clinically doubtful oro-pharyngeal lesions and to compare toluidine blue stain and with the histological evaluation. Key words:Early detection, improved survival, pre-cancer, toluidine blue, vital staining.

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Conflict of interest statement

Conflict of interest statement: The authors have declared that no conflict of interest exist.

Figures

Figure 1
Figure 1
Common site of occurrence of precancerous lesions.
Figure 2
Figure 2
Distribution of habit amongst patients.
Figure 3
Figure 3
Results of toluidine blue staining.
See this image and copyright information in PMC

References

    1. Hegde MC, Kamath PM, Shreedharan S, Dannana NK, Raju RM. Supravital staining: It's role in detecting early malignancies. Indian J Otolaryngology Head Neck Surg. 2006;58:31–4. - PMC - PubMed
    1. Mehrotra R, Singh M, Thomas S, Nair P, Pandya S, Nigam NS. A cross-sectional study evaluating chemiluminescence and autofluorescence in the detection of clinically innocuous precancerous and cancerous oral lesions. J Am Dent Assoc. 2010;141:151–6. - PubMed
    1. Yeole BB, Sankaranarayanan R, Sunny MScL, Swaminathan R, Parkin DM. Survival from head and neck cancer in Mumbai (Bombay), India. Cancer. 2000;89:437–44. - PubMed
    1. Allegra E, Lombardo N, Puzzo L, Garozzo A. The usefulness of toluidine staining as a diagnostic tool for precancerous and cancerous oropharyngeal and oral cavity lesions. Acta Otorhinolaryngol Ital. 2009;29:187–90. - PMC - PubMed
    1. Patton LL, Epstein JB, Kerr AR. Adjunctive techniques for oral cancer examination and lesion diagnosis: a systematic review of the literature. J Am Dent Assoc. 2008;139:896–905. - PubMed

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